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Technically these two antibodies have their
2018-10-20
Technically, these two comt inhibitors have their limitations. Both are better suited for immunofluorescent analysis in frozen tissue sections than in formalin-fixed paraffin-embedded tissues. In addition, anti-Lrig1-VU did not work for immunoprecipitation under our experimental conditions. FACS an
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br Materials and methods br
2018-10-20
Materials and methods Results Discussion In this study, we found that hES membrane transporter express multiple Src-family members, with c-Src, c-Yes, Fyn, Lck and Lyn readily detected at both the transcript and protein levels. Using a phosphospecific antibody for the active form of each k
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It has been proposed that the beneficial
2018-10-20
It has been proposed that the beneficial effects of cell-based therapies might be mediated via several direct and indirect mechanisms, including recruitment of endogenous progenitors, differentiation into functional cardiomyocytes and vascular cells, induction of angiogenesis, promotion of perfusion
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The use of MaSCs from GFP mice for in
2018-10-20
The use of MaSCs from GFP mice for in vivo transplant into wild type recipients enabled us to examine the purity of FACS-gated basal and luminal cell populations from the regenerated GFP glands. All epithelial thyrotropin releasing hormone (GFP positive) were converged in the CD24+ population, whil
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UM171 Supplier Clinical trials with allogeneic neural cells
2018-10-20
Clinical trials with allogeneic neural UM171 Supplier most often involve immunosuppressive treatments (Piccini et al., 2005; Krystkowiak et al., 2007; Sandner et al., 2012), although it is still not fully understood how immunogenic these cells are, whether immunosuppression is a prerequisite and the
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br Materials and methods br Results br
2018-10-20
Materials and methods Results Discussion In this study, we explored the expression pattern and functional significance of GPR56 in the regulation of hematopoietic stem and progenitor maintenance and function during steady-state and stress-induced hematopoiesis. We found that Gpr56 is predom
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br Discussion In this study we obtained
2018-10-20
Discussion In this study, we obtained proof-of-concept data to support our hypothesis that AM can rescue OPC differentiation into mature oligodendrocytes under pathological conditions. Our pharmacological approaches also showed that the AM receptor and PI3K/Akt would mediate these AM effects. AM
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More interestingly our data suggest that SRT
2018-10-20
More interestingly, our data suggest that SRT3025 might suppress p21 transcription through the down-regulation of Egr1. Egr1 modulates p21 gene expression and also plays important functions in HSPC development and proliferation (Krishnaraju et al., 2001; Min et al., 2008; Ragione et al., 2003). With
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We became interested in the potential role of
2018-10-20
We became interested in the potential role of ADF genes and calculating molarity dynamics during osteoblast (OB) differentiation of hMSCs for several reasons. First, actin and microtubules are abundant in osteoblasts (Aubin et al., 1983; Arena et al., 1991). During OB differentiation, cell morpholog
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Recent research suggests that NSCs tropism
2018-10-20
Recent research suggests that NSCs\' tropism towards neoplastic areas is driven by chemoattractants produced at the tumor site. The intratumoral hypoxic microenvironment activates SDF-1/CXCR4 and VEGF/VEGFR signaling pathways, which may act as downstream effectors to increase NSC tropism (Aboody et
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The following are the supplementary data related
2018-10-20
The following are the supplementary data related to this article. Author contribution Acknowledgment We thank Dr. Donald Vander Griend for providing CD133 cDNA, Dr. Oh-Joon Kwon and Ms. Li Zhang for technical help, the technical support by the Cytometry and Cell Sorting Core at Baylor College
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Recently human umbilical cord hUC has been
2018-10-20
Recently, human umbilical cord (hUC) has been investigated as a source of rora with stem/progenitor cell properties. hUC is a desirable source of stem/progenitor cells as it is routinely discarded after delivery, collection is non-invasive and an abundant number of cells are present in cord tissue (
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A microsatellite PCR profile has been
2018-10-20
A microsatellite PCR profile has been obtained for the cell line, and HLA Class I and II typing is available (Table 1). Blood group genotyping gave the blood group O1O1, expected to give rise to blood group O+ (Table 1). The cell line is free from mycoplasma contamination as determined by RT-qPCR.
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Embryonic stem cells ESCs are widely
2018-10-20
Embryonic stem myeloperoxidase (ESCs) are widely used to study early differentiation processes (Posfai et al., 2014). They also hold promise for therapeutic approaches in the field of regenerative medicine (Dressel, 2011; Dressel et al., 2008; Song et al., 2015; Tang and Drukker, 2011). Yet, immune
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br Acknowledgments br Introduction Expression of
2018-10-20
Acknowledgments Introduction Expression of a defined set of transcription factors (OCT4, SOX2, KLF4, and c-MYC) reprograms human somatic cells to a pluripotent state, generating induced pluripotent stem cells (iPSCs) (Park et al., 2008b; Takahashi et al., 2007; Yu et al., 2007). iPSCs are simi
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